Vol. 8, Issue - 1, Part B (2024)

Nargish Marjhan and Md. Abdul Mannan

DOI: https://doi.org/10.33545/2616454X.2024.v8.i1b.267

Abstract:
Traditional medicine and treatments that use plants, animals, or their byproducts in traditional ways can provide relief from suffering for people. More susceptible than other illnesses are microbial infections. Antimicrobial agent from natural sources may be the best remedy in these cases. So, the aim of this study was to examine the antimicrobial activity of methanolic extract of Echinacea pallida to human pathogenic bacteria. Echinacea pallida was collected from the local market then dried in herb drier and mechanically powdered for methanolic extraction by maceration method. Disk diffusion method was used to evaluate the antimicrobial activity of Echinacea pallida extract to Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus spp. by demonstrating zone of inhibition. The extract of Echinacea pallida containing test disk inhibited growth of pathogenic bacteria. Test disk containing 25, 50, 75 and 100 µg showed 8, 10, 11 and 15 mm zone of inhibition against E. coli. 7, 9, 10 and 12 mm zone of inhibition against Staphylococcus aureus. 8, 11, 14 and 16 mm zone of inhibition against Klebsiella pneumoniae. 7, 10, 12 and 14 mm zone of inhibition against Pseudomonas aeruginosa. 100 µg E. pallida containing disk inhibit 9 mm zone whereas 25, 50 and 75 µg E. pallida containing disk were resistance against Proteus spp. Whereas 30 µg Amikacin containing standard antibiotic disk showed 12, 16, 10 and 8 mm zone of inhibition against E. coli, S. aureus, Klebsiella pneumonia and Pseudomonas aeruginosa on the other hand standard 30 µg Amikacin containing disk showed resistance against Proteus spp. respectively. This research concluded that methanolic extract of Echinacea pallida contains antibacterial properties.