Dr. Hidayath Khan Mehdi, Dr. Abdul Saleem and Dr. Zaibunnisa
The Aim of this pre-clinical study is to evaluate the efficacy of Test drug Waj (Acorus calamus) in drug induced hypothyroidism in Albino Wistar Rats.
Material & Methods: The pre-clinical study “Scientific evaluation of efficacy of Waj (A. calamus Linn.) in the management of Qillat-e-Darqia (Hypothyroidism) in Animal experiment.” 30 female albino wistar rats were used in the study. The rats were divided into 06 groups and 03 animals in each group. G1-Control group, G2-Negative group, G3-Standard group, G4-Low test dose, G5-Medium test dose group and G6-High test dose group. Except control group, all the rats were given PTU-10mg/kg/day, oral route for 30 days to induce hypothyroidism. The Hydro-alcoholic extract of Acorus calamus (HAEAC) used for the study. Toxicity study was done according to OECD 423 guidelines for Pilot study. And then the dose of test drug was fixed as Low dose (200mg/kg/bw), Medium dose (400mg/kg/bw) and High dose (600mg/kg/bw).
Results: The effect of Test drug WAJ (HAEAC) in PTU induced hypothyroidism, in high test dose (600mg/kg/bw) results shows the Mean ± SD of T3 is 91.667±2.517 (P < 0.001), T4 is 4.700 ± 0.200 (P < 0.01) and TSH is 26.033 ± 7.061 (P < 0.01).
Conclusion: The present pre-clinical study concluded that the test drug in high test dose (600mg/kg/bw) shows significant results in increasing the thyroid hormone levels T3 & T4 and decreasing the TSH level in PTU induced hypothyroidism in Albino wistar rats